DAO 54:171-173 (2003)  -  doi:10.3354/dao054171

ABSTRACT: Diagnosis of myxosporean Myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp Carassius auratus auratus with a monoclonal antibody, MAb 2D12, raised previously against the parasite. A positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the MAb 2D12. It was also demonstrated that M. rotundus infection can be successfully detected by a simple method, enzyme-linked immunosorbent assay (ELISA), and that skin mucus collected from infected fish skin had a significantly higher optical density (OD) value than that from uninfected fish.

KEY WORDS: Myxosporean · Myxobolus rotundus · Crucian carp · Carassius auratus auratus · Skin mucus · Monoclonal antibody · Diagnosis