AB 30:59-68 (2021)  -  DOI: https://doi.org/10.3354/ab00741

ABSTRACT: This paper presents a novel approach to the long-term recording of electrocardiogram (ECG) signals in prawns, including a 3-lead electrode, a lightweight external wires assembly, a waterproof electrode-wire junction, and a standardized implanting procedure. The proposed low-cost device is easily constructed, even by untrained undergraduate students, using common laboratory materials. Consistent ECG recordings were obtained over a period of 72 h in experiments on 6 freely moving prawns (Macrobrachium rosenbergii). A subsequent experiment conducted continuously for 38 d revealed that despite gradual attenuation the ECG signals could still be identified (signal-to-noise ratio ≥3). The prawns survived implantation and later underwent natural molting, whereupon the electrode was successfully re-implanted. The proposed electrode is a valuable tool by which to gain insight into the long-term physiological state of crustaceans.

KEY WORDS: Electrocardiogram · Crustaceans · Giant river prawn · Macrobrachium rosenbergii · Cardiac arrest

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Cite this article as: Lin HT, Chen TC, Chang JP, Chou ZY, Chiou SY, Tsai ML (2021) Simple method for long-term recording of electrocardiogram signals in prawns. Aquat Biol 30:59-68. https://doi.org/10.3354/ab00741 Export citation Share:    Facebook - - linkedIn