DAO 106:39-47 (2013) - DOI: https://doi.org/10.3354/dao02617
Protection against Vibrio alginolyticus in crimson snapper Lutjanus erythropterus immunized with a DNA vaccine containing the ompW gene
Shuang-Hu Cai1,2,3, Yi-Shan Lu1,2,3, Ji-Chang Jian1,2,3, Bei Wang1,2,3, Yu-Cong Huang1,2,3, Ju-Fen Tang1,2,3, Yu Ding1,2,3, Zao-He Wu2,3,4,*
ABSTRACT: The outer membrane proteins of Vibrio alginolyticus play an important role in the virulence of the bacterium and are potential candidates for vaccine development. In the present study, the ompW gene was cloned, expressed and purified. A DNA vaccine was constructed by inserting the ompW gene into a pcDNA plasmid. Crimson snapper Lutjanus erythropterus (Bloch) were injected intramuscularly with the recombinant plasmid pcDNA-ompW. The expression of the DNA vaccine was detected in gill, head kidney, heart, liver, spleen and injection site muscle of crimson snapper by RT-PCR 7 and 28 d post-vaccination. The ELISA results demonstrated that the DNA vaccine produced an observable antibody response in all sera of the vaccinated fish. In addition, crimson snapper immunized with the DNA vaccine showed a relative percentage survival (RPS) of 92.53%, indicating effective protection against V. alginolyticus infection.
KEY WORDS: Vibriosis · Vaccination · Recombinant plasmid · Outer membrane protein · pcDNA · Aquaculture
| Full text in pdf format | Cite this article as: Cai SH, Lu YS, Jian JC, Wang B and others (2013) Protection against Vibrio alginolyticus in crimson snapper Lutjanus erythropterus immunized with a DNA vaccine containing the ompW gene. Dis Aquat Org 106:39-47. https://doi.org/10.3354/dao02617
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