DAO 123:87-99 (2017)  -  DOI: https://doi.org/10.3354/dao03096

ABSTRACT: Fish die-offs are important signals in tropical marine ecosystems. In 2010, a mass mortality of pufferfish in Hawaii (USA) was dominated by Arothron hispidus showing aberrant neurological behaviors. Using pathology, toxinology, and field surveys, we implicated a series of novel, polar, marine toxins as a likely cause of this mass mortality. Our findings are striking in that (1) a marine toxin was associated with a kill of a fish species that is itself toxic; (2) we provide a plausible mechanism to explain clinical signs of affected fish; and (3) this epizootic likely depleted puffer populations. Whilst our data are compelling, we did not synthesize the toxin de novo, and we were unable to categorically prove that the polar toxins caused mortality or that they were metabolites of an undefined parent compound. However, our approach does provide a template for marine fish kill investigations associated with marine toxins and inherent limitations of existing methods. Our study also highlights the need for more rapid and cost-effective tools to identify new marine toxins, particularly small, highly polar molecules.

KEY WORDS: Dereplication · Pathology · Tetraodontidae · Toxinology · Virology

Full text in pdf format Supplementary material

Cite this article as: Work TM, Moeller PDR, Beauchesne KR, Dagenais J and others (2017) Pufferfish mortality associated with novel polar marine toxins in Hawaii. Dis Aquat Org 123:87-99. https://doi.org/10.3354/dao03096 Export citation Share:    Facebook - - linkedIn