DAO 125:19-29 (2017) - DOI: https://doi.org/10.3354/dao03129
Treatment of Francisella infections via PLGA- and lipid-based nanoparticle delivery of antibiotics in a zebrafish model
Lilia S. Ulanova1,*, Marina Pinheiro2, Carina Vibe1, Claudia Nunes2, Dorna Misaghian3, Steven Wilson3, Kaizheng Zhu3, Federico Fenaroli1, Hanne C. Winther-Larsen4,5, Salette Reis2, Gareth Griffiths1
ABSTRACT: We tested the efficiency of 2 different antibiotics, rifampicin and oxolinic acid, against an established infection caused by fish pathogen Francisella noatunensis ssp. orientalis (F.n.o.) in zebrafish. The drugs were tested in the free form as well as encapsulated into biodegradable nanoparticles, either polylactic-co-glycolic acid (PLGA) nanoparticles or nanostructured lipid carriers. The most promising therapies were PLGA-rifampicin nanoparticles and free oxolinic acid; the PLGA nanoparticles significantly delayed embryo mortality while free oxolinic acid prevented it. Encapsulation of rifampicin in both PLGA and nanostructured lipid carriers enhanced its efficiency against F.n.o. infection relative to the free drug. We propose that the zebrafish model is a robust, rapid system for initial testing of different treatments of bacterial diseases important for aquaculture.
KEY WORDS: Nanoparticles · Francisella orientalis · Infection · Zebrafish embryo · Antibiotics · Danio rerio
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Full text in pdf format Supplementary material | Cite this article as: Ulanova LS, Pinheiro M, Vibe C, Nunes C and others (2017) Treatment of Francisella infections via PLGA- and lipid-based nanoparticle delivery of antibiotics in a zebrafish model. Dis Aquat Org 125:19-29. https://doi.org/10.3354/dao03129
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