DAO 143:139-145 (2021) - DOI: https://doi.org/10.3354/dao03565
NOTE
First case of non-O1/O139 Vibrio cholerae infection in the pink-tailed chalceus Chalceus macrolepidotus in China
Ping Ni, Yi Guo, Jiannan Liu, Hongli Ma, Sicong Guo, Xiaoran Zhao, Shigen Ye*
ABSTRACT: A pathogen was isolated from diseased pink-tailed chalceus Chalceus macrolepidotus during a high-mortality outbreak in a freshwater culture farm in Liaoyang, China. The diseased fish were characterized by disoriented behaviors, exophthalmos, and redness and swelling of the top of the head. A Gram-negative, pure strain of bacteria (CM0428) was isolated from the brain, kidney, and liver. The isolate was identified as Vibrio cholerae based on ompW gene amplification and 16S rRNA and gyrB gene sequence analysis. Serogroup testing indicated that CM0428 was a non-O1/O139 strain of V. cholerae. The challenge test showed that CM0428 exhibited strong virulence to pink-tailed chalceus, and hlyA and toxR virulence-related genes were detected. The isolate was sensitive to multi-class antibiotics, but resistant to tetracycline. Histological examination revealed that V. cholerae CM0428 infection caused multiple organ and tissue lesions, and typical pathological features were cell degeneration and necrosis.
KEY WORDS: Chalceus macrolepidotus · Non-O1/O139 Vibrio cholerae · Pathologic · Outbreak
| Full text in pdf format | Cite this article as: Ni P, Guo Y, Liu J, Ma H, Guo S, Zhao X, Ye S
(2021) First case of non-O1/O139 Vibrio cholerae infection in the pink-tailed chalceus Chalceus macrolepidotus in China. Dis Aquat Org 143:139-145. https://doi.org/10.3354/dao03565
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