DAO 149:25-32 (2022) - DOI: https://doi.org/10.3354/dao03657
Detection of megalocytivirus in Oreochromis niloticus and Pseudoplatystoma corruscans in Brazil
Antônio Augusto Fonseca Jr.*, Mateus Laguardia-Nascimento, Amanda Petrina Scotá Ferreira, Carla Amaral Pinto, Tania Rosária Pereira Freitas, Anselmo Vasconcelos Rivetti Júnior, Valeria Stacchini Ferreira Homem, Marcelo Fernandes Camargos
ABSTRACT: The infectious spleen and kidney necrosis virus (ISKNV) belongs to the genus Megalocytivirus (MCV), a group of double-stranded DNA genome viruses. The aim of this study was to retrospectively analyze samples from suspected foci of MCV infection in freshwater fish in Brazil. Samples were collected from infected fish between 2017 and 2021. Phylogenetic analysis revealed 2 groups of MCV circulating in the country. A genetically homogeneous group formed a clade with ISKNV samples from different parts of the world. Only 2 of the sequences from the state of Goiás showed a small genetic distance when compared to the larger group in the same clade. This study describes the validation of 3 qPCR methods and the presence of MCV in Brazil since 2017, including a genotype not previously described.
KEY WORDS: Megalocytivirus · qPCR · Validation
| Full text in pdf format | Cite this article as: Fonseca AA Jr, Laguardia-Nascimento M, Scotá Ferreira AP, Pinto CA and others (2022) Detection of megalocytivirus in Oreochromis niloticus and Pseudoplatystoma corruscans in Brazil. Dis Aquat Org 149:25-32. https://doi.org/10.3354/dao03657
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