DAO 156:115-121 (2023) - DOI: https://doi.org/10.3354/dao03766
Epidemiological cut-off values for non-O1/ non-O139 Vibrio cholerae disc diffusion data generated by standardised methods
Peter Smith1,*, Laëtitia Le Devendec2, Eric Jouy2, Emeline Larvor2, Jean Lesne3, Alexander K. T. Kirschner4,5,6, Carmen Rehm4,5,6, Melanie Leopold5,6, Sonja Pleininger7, Florian Heger7, Claudia Jäckel8, Cornelia Göllner8, Jonas Nekat8, Jens Andre Hammerl8, Sandrine Baron2
ABSTRACT: This work generates the data needed to set epidemiological cut-off values for disc-diffusion zone measurements of Vibrio cholerae. The susceptibility of 147 European isolates of non-O1/non-O139 V. cholerae to 19 antibiotics was established using a standardised disc diffusion method which specified incubation of Mueller Hinton agar plates at 35°C. Epidemiological cut-off values were calculated by analysis of the zone size data with the statistically based normalised resistance interpretation method. Cut-off values for 17 agents were calculated by analysis of the aggregated data from all 4 laboratories participating in this study. The cut-off values calculated were ≥18 mm for amoxicillin/clavulanate, ≥18 mm for amikacin, ≥19 mm for ampicillin, ≥27 mm for cefepime, ≥31 mm for cefotaxime, ≥24 mm for ceftazidime, ≥24 mm for chloramphenicol, ≥31 mm for ciprofloxacin, ≥16 mm for erythromycin, ≥ 27 mm for florfenicol, ≥16 mm for gentamicin, ≥23 mm for imipenem, ≥25 mm for meropenem, ≥29 mm for nalidixic acid, ≥28 mm for norfloxacin, ≥13 mm for streptomycin and ≥23 mm for tetracycline. For the other 2 agents the data from 1 laboratory was excluded from the censored aggregation because the data from that laboratory was considered excessively imprecise. The cut-off values for these 2 agents calculated for the aggregation of the data from 3 laboratories were ≥23 mm for trimethoprim and ≥24 mm for trimethoprim/sulfamethoxazole. These zone size data will be submitted to the Clinical Laboratory Standards Institute (CLSI) and European Committee for Antimicrobial Susceptibility Testing (EUCAST) for their consideration in setting international consensus epidemiological cut-off values for non O1/non-O139 V. cholerae.
KEY WORDS: Vibrio cholerae · Epidemiological cut-off values · Antimicrobial susceptibility · Disc diffusion · Normalized resistance interpretation
Full text in pdf format  Supplementary Material | Cite this article as: Smith P, Le Devendec L, Jouy E, Larvor E and others (2023) Epidemiological cut-off values for non-O1/ non-O139 Vibrio cholerae disc diffusion data generated by standardised methods. Dis Aquat Org 156:115-121. https://doi.org/10.3354/dao03766
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