Epidemiological cut-off values for Yersina ruckeri disc diffusion data generated by a standardised method

Peter Smith; Laëtitia Le Devendec; Eric Jouy; Emeline Larvor; David Verner-Jeffreys; Andrew Wokorac Joseph; Elliot Stanton; Edel Light; Luana Cortinovis; Tobia Pretto; Amedeo Manfrin; Pierre-Marie Boitard; Matthieu Jamin; Nicolas Keck; Alain Le Breton; Benoit Thuillier; Christian Ravaille; Sandrine Baron

doi:10.3354/dao03779

DAO

Diseases of Aquatic Organisms

via Mailchimp

DAO 158:21-25 (2024) - DOI: https://doi.org/10.3354/dao03779

DAO Special: 'Epidemiological cut off values for aquatic bacteria'

Epidemiological cut-off values for Yersina ruckeri disc diffusion data generated by a standardised method

Peter Smith^1,*, Laëtitia Le Devendec², Eric Jouy², Emeline Larvor², David Verner-Jeffreys^3,4, Andrew Wokorac Joseph^3,4, Elliot Stanton⁵, Edel Light⁶, Luana Cortinovis⁷, Tobia Pretto⁷, Amedeo Manfrin⁷, Pierre-Marie Boitard⁸, Matthieu Jamin⁸, Nicolas Keck⁹, Alain Le Breton¹⁰, Benoit Thuillier¹¹, Christian Ravaille¹², Sandrine Baron²

¹School of Natural Science, University of Galway, Galway H91 TK33 , Ireland
²Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology-Bacteriology-Antimicrobial Resistance Unit, 22440 Ploufragan, France
³Centre for Environment, Fisheries and Aquaculture Science, Weymouth DT4 8UB, UK
⁴UK FAO Reference Centre for AMR, Weymouth DT4 8UB, UK
⁵University of Bristol Veterinary School, Langford, Bristol BS40 5DU, UK
⁶Veterinary Medicines Directorate (VMD),Woodham Lane, New Haw, Surrey KT15 3LS, UK
⁷Fish Pathology Unit, Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
⁸FILI@VET, 15 Rue du Puits, 29600 Saint Martin des Champs, France
⁹Laboratoire Départemental Vétérinaire de l’Hérault, 34000 Montpellier, France
¹⁰Selarl Vet’Eau, 31330 Grenade sur Garonne, France
¹¹Labocéa, Microbiologie vétérinaire, site de Quimper, 29000 Quimper, France
¹²SOCSA Elevage, 81300 Graulhet, France

*Corresponding author: peter.smith@universityofgalway.ie

ABSTRACT: In order to establish the meaning of data generated in antimicrobial agent susceptibility tests, it is necessary to develop internationally harmonised interpretive criteria. Currently, such criteria have not been developed for data generated in studies of the susceptibility of the fish pathogen Yersinia ruckeri. This work generated the data that would be required to set epidemiological cut-off values for the susceptibility data of this species that had been generated using a standardised disc diffusion method that specified the use of Mueller Hinton agar and incubation at 22°C for 24-28 h. Using this method, sets of inhibition zones data for 4 antimicrobial agents were generated by 3 independent laboratories. The data from these laboratories were aggregated and analysed using the statistically based normalised resistance interpretation. For ampicillin, florfenicol, oxytetracycline and trimethoprim-sulfamethoxazole the cut-off values calculated by this analysis were ≥16, ≥23, ≥24 and ≥30 mm, respectively. Evidence is presented demonstrating that the data for these 4 agents was of sufficient quantity and quality that they could be used by the relevant authorities to set internationally harmonised, consensus epidemiological cut-off values for Y. ruckeri.

KEY WORDS: CLSI VET04 · Normalized resistance interpretation · Antimicrobial susceptibility · Disc diffusion · Yersinia ruckeri

Full text in pdf format
Supplementary Material

Cite this article as: Smith P, Le Devendec L, Jouy E, Larvor E and others (2024) Epidemiological cut-off values for Yersina ruckeri disc diffusion data generated by a standardised method. Dis Aquat Org 158:21-25. https://doi.org/10.3354/dao03779

Export citation
Share: Facebook - - linkedIn

Cited by

Previous article Next article