Setting epidemiological cut-off values relevant to MIC and disc diffusion data for Aeromonas salmonicida generated by a standard method

Peter Smith; Elizabeth Buba; Andrew P. Desbois; Alexandra Adams; David Verner-Jeffreys; Andrew Joseph; Edel Light; Laëtitia Le Devendec; Eric Jouy; Emeline Larvor; Pierre-Marie Boitard; Matthieu Jamin; Nicolas Keck; Alain Le Breton; Benoit Thuillier; Christian Ravaille; Thierry Morin; Sandrine Baron

doi:10.3354/dao03798

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DAO 159:29-35 (2024) - DOI: https://doi.org/10.3354/dao03798

DAO Special: 'Epidemiological cut off values for aquatic bacteria'

Setting epidemiological cut-off values relevant to MIC and disc diffusion data for Aeromonas salmonicida generated by a standard method

Peter Smith^1,*, Elizabeth Buba^2,3, Andrew P. Desbois², Alexandra Adams², David Verner-Jeffreys^4,5, Andrew Joseph^4,5, Edel Light³, Laëtitia Le Devendec⁶, Eric Jouy⁶, Emeline Larvor⁶, Pierre-Marie Boitard⁷, Matthieu Jamin⁷, Nicolas Keck⁸, Alain Le Breton⁹, Benoit Thuillier¹⁰, Christian Ravaille¹¹, Thierry Morin¹², Sandrine Baron⁶

¹School of Natural Science, University of Galway, Galway H91 TK33, Ireland
²Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, UK
³Veterinary Medicines Directorate (VMD), Woodham Lane, New Haw, Surrey KT15 3LS, UK
⁴Centre for Environment, Fisheries and Aquaculture Science, Weymouth DT4 8UB, UK
⁵UK FAO Reference Centre for AMR, Weymouth DT4 8UB, UK
⁶Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology-Bacteriology-Antibioresistance Unit, 22440 Ploufragan, France
⁷FILI@VET, 29600 Saint Martin des Champs, France
⁸Laboratoire Départemental Vétérinaire de l’Hérault, 34000 Montpellier, France
⁹Selarl Vet’Eau, 31330 Grenade sur Garonne, France
¹⁰Labocéa, Microbiologie vétérinaire, site de Quimper, 29000 Quimper, France
¹¹SOCSA Elevage, 81300 Graulhet, France
¹²Anses, Ploufragan-Plouzané-Niort Laboratory, Fish Virology, Immunology and Ecotoxicology Unit (VIMEP), 29280 Plouzané, France

*Corresponding author: peter.smith@universityofgalway.ie

ABSTRACT: The Clinical and Laboratory Standards Institute has published epidemiological cut-off values for susceptibility data generated at 22°°C and read after 44-48 h for florfenicol, oxolinic acid and oxytetracycline against Aeromonas salmonicida. The cut-off values for the minimum inhibitory concentration (MIC) and disc diffusion were derived from data obtained by 1 laboratory and 2 laboratories respectively. The present work reports the generation of susceptibility data from additional laboratories and the calculation of provisional cut-off values from aggregations of these data with previously published data. With respect to MIC data, the provisional cut-off values, derived from aggregations of the data from 4 laboratories, were ≤4 µg ml^-1 for florfenicol, ≤0.0625 µg ml^-1 for oxolinic acid and ≤1 µg ml^-1 for oxytetracycline. For disc diffusion data, the provisional cut-off values derived from aggregations of the data from 5 laboratories were ≥30 mm for florfenicol, ≥32 mm for oxolinic acid and ≥25 mm for oxytetracycline. In addition, a cut-off value of ≥29 mm for ampicillin was derived from the aggregation of data from 4 laboratories.

KEY WORDS: Aeromonas salmonicida · Epidemiological cut-off values · Antimicrobial susceptibility · MIC · Minimum inhibitory concentrations · Disc diffusion · Normalised resistance interpretation · ECOFFinder

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Cite this article as: Smith P, Buba E, Desbois AP, Adams A and others (2024) Setting epidemiological cut-off values relevant to MIC and disc diffusion data for Aeromonas salmonicida generated by a standard method. Dis Aquat Org 159:29-35. https://doi.org/10.3354/dao03798

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