Epidemiological cut-off values for Vibrio parahaemolyticus calculated from minimal inhibitory concentration data generated at 35 and 28°C

Peter Smith; Andrew Joseph; Craig Baker-Austin; Nisha Kang; Sandrine Baron; Laëtitia Le Devendec; Eric Jouy; Thomas Chisnall; Alistair R. Davies; Stefan Schwarz; Andrea T. Feßler; Tanja Ahrens; Johanna Jahnen; Thomas Alter; Susanne Fleischmann; Jens Andre Hammerl; Claudia Jäckel; Charles M. Gieseker; Tina C. Crosby; Elliott C. Kittel; Ron A. Miller; Trevor Alexander; Kayleigh Carranza; Claire B. Burbick; Biyun Ching; Jun Heng Soh; You Rong Chng; Wai Kwan Wong; Charlene J. Fernandez; Siow Foong Chang; David Verner-Jeffreys; Andy Powell

doi:10.3354/dao03831

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Diseases of Aquatic Organisms

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DAO 160:127-134 (2024) - DOI: https://doi.org/10.3354/dao03831

DAO Special: 'Epidemiological cut off values for aquatic bacteria'

Epidemiological cut-off values for Vibrio parahaemolyticus calculated from minimal inhibitory concentration data generated at 35 and 28°C

Peter Smith^1,*, Andrew Joseph^2,3, Craig Baker-Austin^2,3, Nisha Kang^2,3, Sandrine Baron⁴, Laëtitia Le Devendec⁴, Eric Jouy⁴, Thomas Chisnall⁵, Alistair R. Davies⁵, Stefan Schwarz^6,7, Andrea T. Feßler^6,7, Tanja Ahrens^6,7, Johanna Jahnen^6,7, Thomas Alter⁸, Susanne Fleischmann⁸, Jens Andre Hammerl⁹, Claudia Jäckel⁹, Charles M. Gieseker¹⁰, Tina C. Crosby¹⁰, Elliott C. Kittel¹⁰, Ron A. Miller¹⁰, Trevor Alexander¹¹, Kayleigh Carranza¹¹, Claire B. Burbick¹¹, Biyun Ching¹², Jun Heng Soh¹², You Rong Chng¹², Wai Kwan Wong¹², Charlene J. Fernandez¹², Siow Foong Chang¹², David Verner-Jeffreys¹³, Andy Powell^2,3

¹School of Natural Science, University of Galway, Galway H91 TK33, Ireland
²Centre for Environment, Fisheries and Aquaculture Science, Weymouth DT4 8UB, UK
³UK FAO Reference Centre for AMR, Weymouth DT4 8UB, UK
⁴Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology Bacteriology Antimicrobial Resistance, 22440 Ploufragan, France
⁵Animal and Plant Health Agency, New Haw Addlestone, Surrey KT15 3NB, UK
⁶Institute of Microbiology and Epizootics, Centre of Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany
⁷Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany
⁸Institute of Food Safety and Food Hygiene, Centre of Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany
⁹Consultant Laboratory for Vibrio spp. in Food, Department Biological Safety, German Federal Institute for Risk Assessment, 1089 Berlin, Germany
¹⁰Center for Veterinary Medicine, US Food and Drug Administration, Laurel, Maryland 20708, USA
¹¹Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, Washington 99163, USA
¹²Animal and Veterinary Service, National Parks Board, Singapore 259569
¹³WorldFish, Jalan Batu Maung, 11960 Bayan Lepas, Penang, Malaysia

*Corresponding author: peter.smith@universityofgalway.ie

ABSTRACT: This work was performed to generate the data needed to set epidemiological cut-off values for minimal inhibitory concentrations (MICs) of 10 antimicrobial agents against Vibrio parahaemolyticus determined using standardised broth microdilution protocols. Eight laboratories performed broth microdilution tests with incubation at 35°C for 16 to 20 h, and 7 also performed tests on the same isolates with incubation at 28°C for 24 to 28 h. Data were analysed by the ECOFFinder and normalised resistance interpretation algorithms. The cut-off values calculated for ceftazidime, florfenicol and trimethoprim/sulfamethoxazole, 1, 1 and 0.25/4.75 µg ml^-1, respectively, were the same when calculated from data obtained at both temperatures. The cut-off values calculated from data obtained at 35°C and from data obtained at 28°C were 0.25 and 0.5 µg ml^-1 for enrofloxacin, 2 and 4 µg ml^-1 for gentamicin, 0.5 and 1 µg ml^-1 for oxolinic acid and 2 and 1 µg ml^-1 for oxytetracycline, respectively. The influence of incubation temperature on MIC values was investigated by comparing MICs obtained at 35 and 28°C for a specific antimicrobial agent with a particular isolate by an individual laboratory. Results showed that 56% of 1473 of these paired MIC values were identical, while 38% differed from one another by not more than 1 dilution step. The data generated in this work will be submitted to the Clinical and Laboratory Standards Institute for consideration in their setting of internationally agreed epidemiological cut-off values for V. parahaemolyticus that are essential for interpreting antimicrobial susceptibility testing data of this species.

KEY WORDS: Vibrio parahaemolyticus · Epidemiological cut-off values · Minimal inhibitory concentration · Incubation temperature · MIC · CO^WT · Antimicrobial

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Cite this article as: Smith P, Joseph A, Baker-Austin C, Kang N and others (2024) Epidemiological cut-off values for Vibrio parahaemolyticus calculated from minimal inhibitory concentration data generated at 35 and 28°C. Dis Aquat Org 160:127-134. https://doi.org/10.3354/dao03831

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