DAO 161:1-9 (2025) - DOI: https://doi.org/10.3354/dao03833
Development of colloidal gold immunochromatographic strip for rapid detection of cyvirus cyprinidallo 2
Lupin Zhao1,#, Jiaying Wang1,#, Xiaoru Liu1, Yi Zhou1, Jiaxun Li1, Liqun Lu1,2,3, Yousheng Jiang1,2,3,4,*
#These authors contributed equally to this work
ABSTRACT: Cyvirus cyprinidallo 2 (CyHV-2) is the pathogen of herpesviral hematopoietic necrosis (HVHN) that mainly infects goldfish Carassius auratus and crucian carp C. carassius and is characterized by high infectivity and pathogenicity. The availability of rapid and convenient detection methods is essential for early detection of CyHV-2. A colloidal gold immunochromatographic strip using 2 specific anti-CyHV-2 monoclonal antibodies (MAbs) has been developed and validated for rapid detection of CyHV-2. The test results can be viewed within 10min. The detection limit of test strip was 2.08 × 102 TCID50 ml-1, and it showed no cross-reactivity with other freshwater fish viral pathogen except KHV (koi herpesvirus). The specificity of the strip was 100% when spleen and kidney tissues of CyHV-2 infected and healthy crucian carp were assayed following an experimental challenge. The colloidal gold immunochromatographic strip will be an effective device for the rapid detection of CyHV-2 in the future.
KEY WORDS: CyHV-2 · Monoclonal antibodies · Rapid detection · Colloidal gold immunochromatography assay · GICA
| Full text in pdf format | Cite this article as: Zhao L, Wang J, Liu X, Zhou Y, Li J, Lu L, Jiang Y
(2025) Development of colloidal gold immunochromatographic strip for rapid detection of cyvirus cyprinidallo 2. Dis Aquat Org 161:1-9. https://doi.org/10.3354/dao03833
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