DAO 33:73-75 (1998) - doi:10.3354/dao033073
Generation and preliminary characterisation of monoclonal antibodies directed to glycerophospholipid:cholesterol acyltransferase (GCAT) native epitopes of Aeromonas salmonicida
Ingolf Lachmann*, Ulrich Wagner, Dietlind Hädge, Karl Drössler
*E-mail: ilachman@server1.rz.uni-leipzig.de
Four monoclonal antibodies (MAbs) directed to native glycerophospholipid:cholesterol acyltransferase (GCAT) epitopes of Aeromonas salmonicida were isolated using an esterase capture assay. The molecular mass of this MAb-defined antigen was estimated to be 26 kDa in SDS-PAGE. Three different epitope specificities of these MAbs were demonstrated. It was shown that all 4 MAbs recognize GCAT in culture filtrates of the strain MT004 excluding the simultaneous trapping of other components. None of the MAbs react with the denatured GCAT in Western blots.
Aeromonas salmonicida · GCAT · Monoclonal antibodies
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