Lectin-reactive components of the microsporidian Glugea 
plecoglossi and their relation to spore phagocytosis by head kidney macrophages of ayu Plecoglossus altivelis

Jeong-Ho Kim; Kazuo Ogawa; Hisatsugu Wakabayashi

doi:10.3354/dao039059

DAO

Diseases of Aquatic Organisms

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DAO 39:59-63 (1999) - doi:10.3354/dao039059

Lectin-reactive components of the microsporidian Glugea plecoglossi and their relation to spore phagocytosis by head kidney macrophages of ayu Plecoglossus altivelis

Jeong-Ho Kim*, Kazuo Ogawa, Hisatsugu Wakabayashi

Department of Aquatic Bioscience, Graduate School of Agriculture and Agricultural Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan

*Present address: Laboratory of Aquatic Animal Diseases, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungcheongbuk-do, 360-763, Korea. E-mail: kimjh89@yahoo.com

ABSTRACT: We investigated the reactivity of lectins to spores of Glugea plecoglossi from ayu Plecoglossus altivelis. Smear preparations of purified spores were treated with 8 kinds of lectins. Lectin blots were used to detect glycoproteins of spore lysates. In addition, lectin-treated spores were applied to head kidney macrophages of ayu, and the percentage of phagocytosis (PP) was calculated and compared with the control. Two lectins (ConA, WGA) reacted with the surface of the spores, and a major band (55 kDa) and some minor bands were visualized on blots after treatment with these. PP was decreased after ConA treatment. From these results, we suggest that G. plecoglossi spores can be phagocytized by ayu head kidney macrophages via ConA-reactive glycoprotein-mediated recognition.

KEY WORDS: Glugea

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Published in DAO Vol. 39, No. 1. Publication date: December 22, 1999
Print ISSN:0177-5103; Online ISSN:1616-1580
Copyright © 1999 Inter-Research.
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