Identification of fish-parasitic Myxobolus (Myxosporea) species using a combined  PCR-RFLP method

Edit Eszterbauer; Mária Benkó´; Ádám Dán; Kálmán Molnár

doi:10.3354/dao044035

DAO

Diseases of Aquatic Organisms

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DAO 44:35-39 (2001) - doi:10.3354/dao044035

Identification of fish-parasitic Myxobolus (Myxosporea) species using a combined PCR-RFLP method

Edit Eszterbauer^1,*, Mária Benkó´¹, Ádám Dán^1,2, Kálmán Molnár¹

¹Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, 1581 Budapest, Hungary
²Central Veterinary Institute, Tábornok u. 2, 1149 Budapest, Hungary

*E-mail: eedit@novell.vmri.hu

ABSTRACT: Polymerase chain reaction (PCR) with primers specific for the family Myxobolidae was used to amplify a part of the 18S ribosomal RNA gene of Myxobolus species. The length of the amplified fragments was approximately 1600 base pairs. Six Myxobolus species identified on the basis of morphological features were compared using a combined PCR-RFLP method. The cleavage patterns generated by 2 frequent cutter restriction enzymes (HinfI and MspI) were suitable for the differentiation of the examined Myxobolus species.

KEY WORDS: Myxobolus spp. · Myxosporea · Fish parasite · PCR · RFLP

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DAO 44:35-39 (2001) - doi:10.3354/dao044035

Identification of fish-parasitic Myxobolus (Myxosporea) species using a combined PCR-RFLP method

Edit Eszterbauer1,*, Mária Benkó´1, Ádám Dán1,2, Kálmán Molnár1

Edit Eszterbauer^1,*, Mária Benkó´¹, Ádám Dán^1,2, Kálmán Molnár¹