Purification of Piscirickettsia salmonis and  associated phage particles

Sema Akinlar Yuksel; Kim D. Thompson; Anthony E. Ellis; Alexandra Adams

doi:10.3354/dao044231

DAO

Diseases of Aquatic Organisms

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DAO 44:231-235 (2001) - doi:10.3354/dao044231

Purification of Piscirickettsia salmonis and associated phage particles

Sema Akinlar Yuksel¹, Kim D. Thompson^1,*, Anthony E. Ellis², Alexandra Adams¹

¹Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, Scotland, UK
²RS Marine Laboratory, Victoria Road, Aberdeen AB11 9DB, Scotland, UK

*Corresponding author. E-mail: kdt1@stir.ac.uk

ABSTRACT: Piscirickettsia salmonis was isolated from cell culture using differential centrifugation and purified on a 30% Percol gradient. The purity of the preparation was assessed by transmission electron microscopy and phage-like particles were found to be associated with some of the P. salmonis isolates examined. This is believed to be the first report of a phage associated with rickettsia from fish.

KEY WORDS: Piscirickettsia salmonis · Rickettsia · Purification · Bacteriophage

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DAO 44:231-235 (2001) - doi:10.3354/dao044231

Purification of Piscirickettsia salmonis and associated phage particles

Sema Akinlar Yuksel1, Kim D. Thompson1,*, Anthony E. Ellis2, Alexandra Adams1

Sema Akinlar Yuksel¹, Kim D. Thompson^1,*, Anthony E. Ellis², Alexandra Adams¹