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Diseases of Aquatic Organisms

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DAO 48:233-236 (2002)  -  doi:10.3354/dao048233

Multiple viral infection in Penaeus monodon shrimp postlarvae in an Indian hatchery

Subramaniyan Manivannan, Subhendu K. Otta, Indrani Karunasagar, Iddya Karunasagar*

Department of Fishery Microbiology, University of Agricultural Sciences, College of Fisheries, Mangalore 575 002, India
*Corresponding author. E-mail:

ABSTRACT: Moribund Penaeus monodon postlarvae (PL8-PL10) in a hatchery in India were found to be simultaneously infected by 3 different viruses. They were highly infected with monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) by histology and with white spot syndrome virus (WSSV) by non-nested polymerase chain reaction (PCR). Apparently healthy postlarvae tested from the same hatchery were positive for MBV and WSSV by nested PCR only. Tissue sections of such postlarvae did not show any histopathological changes. The simultaneous occurrence of these 3 viruses in hatchery-reared postlarval P. monodon is being reported for the first time.


KEY WORDS: MBV · HPV · WSSV · Shrimp postlarvae · Penaeus monodon · Mortality · PCR · Histopathology


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