DAO 53:11-13 (2003) - doi:10.3354/dao053011
Highly conserved sequences of three major virion proteins of a Korean isolate of white spot syndrome virus (WSSV)
Chang Hoon Moon1, Jeong Wan Do2, Seung Ju Cha2, Won Joon Yoon1, Seong Bum Kim1, Myoung Seok Ko1, Myoung Ae Park2, Jin Woo Kim2, Sang Kyu Sohn2, Jong Hwa Lee3, Jeong Woo Park1,*
ABSTRACT: In Korea, mass mortality occurred among cultured shrimp with visible macroscopic white spots in 2000, and we confirmed the presence of white spot syndrome virus (WSSV) in the tissues of moribund shrimp by electron microscopy. In order to identify the characteristics of this Korean isolate of WSSV, we cloned and characterized its genomic DNA coding for VP24, VP26, and VP28. On the nucleotide level, VP24, VP26, and VP28 of the Korean isolate were found to be 100%, 100%, and 99% identical to those of Taiwan, Thailand and Chinese isolates, respectively. On the deduced amino-acid level, all 3 virion proteins showed 100% identity to those of the foreign isolates. The extent of sequence identity suggests that the Korean isolate originated from the same ancestor as the Taiwanese, Thai and Chinese isolates.
KEY WORDS: White spot syndrome virus · Korean WSSV isolates · Conserved virion proteins
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