DAO 69:249-253 (2006) - doi:10.3354/dao069249
Polyclonal antibodies specific for VP1 and VP3 capsid proteins of Taura syndrome virus (TSV) produced via gene cloning and expression
Parin Chaivisuthangkura1, Thanawan Tejangkura1, Sombat Rukpratanporn2, Siwaporn Longyant1, Weerawan Sithigorngul1, Paisarn Sithigorngul1,*
ABSTRACT: Capsid protein genes VP1 and VP3 of Taura syndrome virus (TSV) were cloned into pGEX-6P-1 expression vector and transformed into Escherichia coli BL21. After induction, recombinant VP1 (rVP1) and recombinant VP3 (rVP3) were produced, purified by SDS-PAGE and used for immunization of Swiss mice for antisera production. Anti-rVP1 and anti-rVP3 antisera showed specific immunoreactivities to rVP1 and rVP3 proteins, respectively, by Western blot assay and also yielded good results for detection of TSV in various shrimp tissues by immunohistochemistry. This is the first step towards our target of preparing monoclonal antibodies specific to rVP1 and rVP3 for use in simple immuno-diagnostic test kits for TSV detection and identification.
KEY WORDS: Immunohistochemistry · Litopenaeus vannamei · Polyclonal antibody · VP1 · VP3 · Western blot · Taura syndrome virus · TSV
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