DAO 76:163-168 (2007) - doi:10.3354/dao076163
Genotyping spring viraemia of carp virus and other piscine vesiculo-like viruses using reverse hybridisation
A. M. Sheppard, R.-M. Le Deuff, P. D. Martin, G. Woolford, K. Way, D. M. Stone*
ABSTRACT: A simple nylon membrane-based DNA macroarray was developed to genotype spring viraemia of carp virus (SVCV) and related viruses. Twenty-six viruses were genotyped using the array, and the results were confirmed by phylogenetic analysis of a 426 bp partial glycoprotein gene sequence. The array was not only capable of discriminating between the 4 main genogroups of cyprinid vesiculo-type viruses described previously, but also accurately sub-type the SVC viruses assigned to Genogroup I. The assay offers a practical solution for diagnostic laboratories that currently lack a sequencing capability to confirm the nature of PCR products generated in suspected SVCV cases.
KEY WORDS: Rhabdovirus · Spring viraemia of carp virus · SVCV · Vesiculovirus · Genotyping · Reverse hybridisation
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