DAO 82:105-109 (2008) - DOI: https://doi.org/10.3354/dao01972
Real-time PCR detection of Moritella viscosa, the likely causal agent of winter-ulcer in Atlantic salmon Salmo salar and rainbow trout Oncorhynchus mykiss
S. Grove1,*, L. J. Reitan1, T. Lunder2, D. Colquhoun1
ABSTRACT: We report the development of a real-time PCR protocol for specific detection of Moritella viscosa. This bacterium is considered to be the main aetiological agent in development of winter-ulcer, a disease severely affecting salmonid aquaculture in Norway. From a newly elaborated draft version of the genome of M. viscosa, the tonB gene sequence was selected as a suitable basis for the development of the real-time PCR assay. The real-time PCR demonstrated the presence of M. viscosa DNA sequences in 88.1% of samples collected from 35 outbreaks of winter-ulcer in Norwegian fish farms. In contrast, standard culturing on blood agar identified M. viscosa in only 39.7% of fish. While the culturing method revealed a similar prevalence (26 to 27%) of M. viscosa in kidney and ulcer samples, substantially more ulcer (81.5%) than kidney (49.7%) samples were shown positive by real-time PCR.
KEY WORDS: Moritella viscosa · Winter-ulcer · Aquaculture · Salmonids · Norway · Real-time PCR
| Full text in pdf format | Cite this article as: Grove S, Reitan LJ, Lunder T, Colquhoun D
(2008) Real-time PCR detection of Moritella viscosa, the likely causal agent of winter-ulcer in Atlantic salmon Salmo salar and rainbow trout Oncorhynchus mykiss. Dis Aquat Org 82:105-109. https://doi.org/10.3354/dao01972
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