DAO 84:209-217 (2009) - DOI: https://doi.org/10.3354/dao02045
Identification of Vibrio harveyi isolated from diseased cultured wedge sole Dicologoglossa cuneata
Jose R. López1,*, Elena de la Roca1, Soledad Núñez2, Roberto de la Herran3, José I. Navas1, Manuel Manchado4, Marcelino Herrera1, Alicia E. Toranzo2
ABSTRACT: We report the first isolation of Vibrio harveyi from wedge sole Dicologoglossa cuneata. The pathogen was recovered from ulcers and internal organs of ailing cultured fish, from 7 different outbreaks between 2004 and 2006. The 15 isolates found were phenotypically characterized using biochemical tests and BIOLOG GN plates, which revealed high phenotypic diversity. Diagnosis was confirmed with PCR using V. harveyi specific primers and partial 16S and 23S rRNA gene sequencing. A virulence evaluation of the isolates was also performed using fry and juvenile wedge sole. Significant mortalities were recorded by intraperitoneal injection; however, no mortalities were recorded by bath immersion.
KEY WORDS: Vibrio harveyi · Characterization · Wedge sole · Dicologoglossa cuneata · Virulence
| Full text in pdf format | Cite this article as: López JR, de la Roca E, Núñez S, de la Herran R and others (2009) Identification of Vibrio harveyi isolated from diseased cultured wedge sole Dicologoglossa cuneata. Dis Aquat Org 84:209-217. https://doi.org/10.3354/dao02045 Export citation Share: Facebook - - linkedIn |
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