DAO 93:83-91 (2010) - DOI: https://doi.org/10.3354/dao02268
Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species
Yu Rao1,2, Liqiao Zhong1,2, Tao Liao1, Shiwei Jin1, Yuzhen Wang1, Bo Song1, Jia Li1,2, Xiaohua Zhang1, Sean M. Hemmingsen3, Ying Xu1, Heping Dai1,*
ABSTRACT: Various polyclonal and monoclonal antibodies have been developed for vitellogenin (Vtg) bioassays in different aquatic species. Preparation of these reagents is time-consuming and expensive. In the present study, a phage-displayed, recombinant, single-chain variable fragment (scFv) format antibody library was constructed using splenic mRNA from non-immunized mice. After 3 rounds of panning, 3 scFv antibodies with specificity for the highly conserved N-terminal region of cyprinid fish Vtg were isolated. One of these, antibody H4, bound purified Vtg from common carp Cyprinus carpio, zebrafish Danio rerio and Chinese rare minnow Gobiocypris rarus with similar affinities and detected Vtg in zebrafish plasma samples. This study provides a simple, low cost Vtg bioassay for plasma samples from a variety of cyprinid fish.
KEY WORDS: Vitellogenin detection · Cyprinid fish · Phage display · ScFv antibody · Bioassay · Binding affinity
| Full text in pdf format | Cite this article as: Rao Y, Zhong L, Liao T, Jin S and others (2010) Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species. Dis Aquat Org 93:83-91. https://doi.org/10.3354/dao02268 Export citation Share: Facebook - - linkedIn |
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