DAO 94:9-16 (2011) - DOI: https://doi.org/10.3354/dao02311
Identification of a 27.8 kDa protein from flounder gill cells involved in lymphocystis disease virus binding and infection
Mu Wang, Xiu-Zhen Sheng*, Jing Xing, Xiao-Qian Tang, Wen-Bin Zhan
ABSTRACT: In vitro, lymphocystis disease virus (LCDV) infection of flounder gill (FG) cell cultures causes obvious cytopathic effect (CPE). We describe attempts to isolate and characterize the LCDV-binding molecule(s) on the plasma membrane of FG cells that were responsible for virus entry. The results showed that the co-immunoprecipitation assay detected a 27.8 kDa molecule from FG cells that bound to LCDV. In a blocking ELISA, pre-incubation of FG cell membrane proteins with the specific antiserum developed against the 27.8 kDa protein could block LCDV binding. Similarly, antiserum against 27.8 kDa protein could also inhibit LCDV infection of FG cells in vitro. Mass spectrometric analysis established that the 27.8 kDa protein and β-actin had a strong association. These results strongly supported the possibility that the 27.8 kDa protein was the putative receptor specific for LCDV infection of FG cells.
KEY WORDS: Lymphocystis disease virus · Receptor · Virus entry · Flounder gill cells · Co-immunoprecipitation
| Full text in pdf format | Cite this article as: Wang M, Sheng XZ, Xing J, Tang XQ, Zhan WB
(2011) Identification of a 27.8 kDa protein from flounder gill cells involved in lymphocystis disease virus binding and infection. Dis Aquat Org 94:9-16. https://doi.org/10.3354/dao02311
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